Cargando…

Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A(1) receptor ligands

BACKGROUND AND PURPOSE: The introduction of fluorescence-based techniques, and in particular the development of fluorescent ligands, has allowed the study of G protein-coupled receptor pharmacology at the single cell and single molecule level. This study evaluated how the physicochemical nature of t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Baker, Jillian G, Middleton, Richard, Adams, Luke, May, Lauren T, Briddon, Stephen J, Kellam, Barrie, Hill, Stephen J
Formato:	Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 2010
Materias:	Themed Section: Research Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829203/ https://www.ncbi.nlm.nih.gov/pubmed/20105183 http://dx.doi.org/10.1111/j.1476-5381.2009.00488.x

Ejemplares similares

Synthesis and Characterization of High-Affinity 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene-Labeled Fluorescent Ligands for Human β-Adrenoceptors
por: Baker, Jillian G., et al.
Publicado: (2011)

Fluorescence‐ and bioluminescence‐based approaches to study GPCR ligand binding
por: Stoddart, Leigh A, et al.
Publicado: (2015)

Fragment Screening at Adenosine-A(3) Receptors in Living Cells Using a Fluorescence-Based Binding Assay
por: Stoddart, Leigh A., et al.
Publicado: (2012)

NanoBiT Complementation to Monitor Agonist-Induced Adenosine A(1) Receptor Internalization
por: Soave, Mark, et al.
Publicado: (2019)

Optimization of Peptide Linker-Based Fluorescent Ligands for the Histamine H(1) Receptor
por: Kok, Zhi Yuan, et al.
Publicado: (2022)

Effect of a toggle switch mutation in TM6 of the human adenosine A(3) receptor on Gi protein-dependent signalling and Gi-independent receptor internalization
por: Stoddart, Leigh A, et al.
Publicado: (2014)

Kinetic analysis of antagonist-occupied adenosine-A(3) receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism
por: Corriden, Ross, et al.
Publicado: (2014)

Pharmacology under the microscope: the use of fluorescence correlation spectroscopy to determine the properties of ligand–receptor complexes
por: Briddon, Stephen J., et al.
Publicado: (2007)

Adenosine-A(3) receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes
por: Corriden, Ross, et al.
Publicado: (2013)

Small-Molecule Fluorescent Ligands for the CXCR4 Chemokine Receptor
por: Dekkers, Sebastian, et al.
Publicado: (2023)

Efficient G protein coupling is not required for agonist‐mediated internalization and membrane reorganization of the adenosine A(3) receptor
por: Stoddart, Leigh A., et al.
Publicado: (2021)

A Non-imaging High Throughput Approach to Chemical Library Screening at the Unmodified Adenosine-A(3) Receptor in Living Cells
por: Arruda, Maria Augusta, et al.
Publicado: (2017)

Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2‐adrenoceptor in CRISPR/Cas9 genome‐edited HEK293T cells at low expression levels
por: Goulding, Joëlle, et al.
Publicado: (2021)

Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells
por: Stoddart, Leigh A., et al.
Publicado: (2020)

Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes
por: Králová, Jarmila, et al.
Publicado: (2020)

Development of novel fluorescent histamine H(1)-receptor antagonists to study ligand-binding kinetics in living cells
por: Stoddart, Leigh A., et al.
Publicado: (2018)

Allosteric interactions across native adenosine-A(3) receptor homodimers: quantification using single-cell ligand-binding kinetics
por: May, Lauren T., et al.
Publicado: (2011)

Interactions between malaria and human immunodeficiency virus anno 2014
por: Van geertruyden, J-P
Publicado: (2014)

Endophthalmitis
por: Durand, M L
Publicado: (2013)

Skirts as Flags: Transitional Justice, Gender and Everyday Nationalism in Kosovo
por: Krasniqi, Vjollca, et al.
Publicado: (2020)

Modelling in infectious diseases: between haphazard and hazard
por: Neuberger, A., et al.
Publicado: (2013)

Web‐based participatory surveillance of infectious diseases: the Influenzanet participatory surveillance experience
por: Paolotti, D., et al.
Publicado: (2013)

The microarray technology: facts and controversies
por: Lévêque, N., et al.
Publicado: (2012)

Biological warfare, bioterrorism, and biocrime
por: Jansen, H. J., et al.
Publicado: (2014)

Applying proteomic technology to clinical virology
por: Mancone, C., et al.
Publicado: (2012)

The global burden of bacterial and viral zoonotic infections
por: Christou, L.
Publicado: (2011)

Twenty years of cell-penetrating peptides: from molecular mechanisms to therapeutics
por: Heitz, Frederic, et al.
Publicado: (2009)

What We Do Not Know About the Costs of Immunization Programs in Low- and Middle-Income Countries
por: Portnoy, Allison, et al.
Publicado: (2021)

Molecular regulation and pharmacological targeting of the β‐catenin destruction complex
por: van Kappel, Eline C, et al.
Publicado: (2017)

Identification and structure elucidation of the pro‐resolving mediators provides novel leads for resolution pharmacology
por: Dalli, Jesmond, et al.
Publicado: (2018)

The GPCR-associated sorting protein 1 regulates ligand-induced down-regulation of GPR55
por: Kargl, J, et al.
Publicado: (2012)

Integrating cardiomyocytes from human pluripotent stem cells in safety pharmacology: has the time come?
por: Sala, Luca, et al.
Publicado: (2016)

Decisional Regret in Long-Term Australian Allogeneic Hematopoietic Stem Cell Transplantation Survivors: A Cross-Sectional Survey
por: McErlean, Gemma, et al.
Publicado: (2023)

Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies
por: Soave, Mark, et al.
Publicado: (2020)

Three‐dimensional electron microscopy techniques for unravelling mitochondrial dysfunction in heart failure and identification of new pharmacological targets
por: Daghistani, Hussam M, et al.
Publicado: (2018)

A live cell NanoBRET binding assay allows the study of ligand-binding kinetics to the adenosine A(3) receptor
por: Bouzo-Lorenzo, Monica, et al.
Publicado: (2019)

Negative cooperativity across β(1)-adrenoceptor homodimers provides insights into the nature of the secondary low-affinity CGP 12177 β1-adrenoceptor binding conformation
por: Gherbi, Karolina, et al.
Publicado: (2015)

Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling
por: Zhang, Lei, et al.
Publicado: (2022)

Signalling pathways underlying structural plasticity of dendritic spines
por: Patterson, Michael, et al.
Publicado: (2011)

NOX1, 2, 4, 5: counting out oxidative stress
por: Wingler, K, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_uld4r0h2enal35mqqtqqet3rc8