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Application of PCR amplicon sequencing using a single primer pair in PCR amplification to assess variations in Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs

BACKGROUND: The presence of various EPIYA tyrosine phosphorylation motifs in the CagA protein of Helicobacter pylori has been suggested to contribute to pathogenesis in adults. In this study, a unique PCR assay and sequencing strategy was developed to establish the number and variation of cagA EPIYA...

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Detalles Bibliográficos
Autores principales: Monstein, Hans-Jürg, Karlsson, Anneli, Ryberg, Anna, Borch, Kurt
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829584/
https://www.ncbi.nlm.nih.gov/pubmed/20181142
http://dx.doi.org/10.1186/1756-0500-3-35