Serious Overestimation in Quantitative PCR by Circular (Supercoiled) Plasmid Standard: Microalgal pcna as the Model Gene

Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard. A recent study showed that supercoiled circular confirmation of DNA appeared to su...

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Detalles Bibliográficos
Autores principales: Hou, Yubo, Zhang, Huan, Miranda, Lilibeth, Lin, Senjie
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832698/
https://www.ncbi.nlm.nih.gov/pubmed/20221433
http://dx.doi.org/10.1371/journal.pone.0009545

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832698/
https://www.ncbi.nlm.nih.gov/pubmed/20221433
http://dx.doi.org/10.1371/journal.pone.0009545

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