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Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 was cloned and overexpressed in Escherichia coli. The enzyme was purified fivefold by membrane filtration and ion exchange. The purified enzyme had a V (max) of 2.03 × 10(−9) s (−1) and a K (m) of 206 μM on guanosine. T...

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Detalles Bibliográficos
Autores principales: Visser, Daniel F., Hennessy, Fritha, Rashamuse, Konanani, Louw, Maureen E., Brady, Dean
Formato: Texto
Lenguaje:English
Publicado: Springer Japan 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832885/
https://www.ncbi.nlm.nih.gov/pubmed/20063024
http://dx.doi.org/10.1007/s00792-009-0297-4