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Evaluating the efficacy of tryptophan fluorescence and absorbance as a selection tool for identifying protein crystals

The environment of individual tryptophans in known protein structures and the effectiveness of four commercial robotic UV microscopes to illuminate tryptophan-containing protein crystals by either tryptophan fluorescence (epi-illumination) or absorbance (transmission) are evaluated. In agreement wit...

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Detalles Bibliográficos
Autor principal: Gill, Harindarpal S.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833058/
https://www.ncbi.nlm.nih.gov/pubmed/20208182
http://dx.doi.org/10.1107/S1744309110002022