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Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases
During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We develo...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835940/ https://www.ncbi.nlm.nih.gov/pubmed/20212316 http://dx.doi.org/10.1083/jcb.200909005 |
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author | Jaqaman, Khuloud King, Emma M. Amaro, Ana C. Winter, Jennifer R. Dorn, Jonas F. Elliott, Hunter L. Mchedlishvili, Nunu McClelland, Sarah E. Porter, Iain M. Posch, Markus Toso, Alberto Danuser, Gaudenz McAinsh, Andrew D. Meraldi, Patrick Swedlow, Jason R. |
author_facet | Jaqaman, Khuloud King, Emma M. Amaro, Ana C. Winter, Jennifer R. Dorn, Jonas F. Elliott, Hunter L. Mchedlishvili, Nunu McClelland, Sarah E. Porter, Iain M. Posch, Markus Toso, Alberto Danuser, Gaudenz McAinsh, Andrew D. Meraldi, Patrick Swedlow, Jason R. |
author_sort | Jaqaman, Khuloud |
collection | PubMed |
description | During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion. |
format | Text |
id | pubmed-2835940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28359402010-09-08 Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases Jaqaman, Khuloud King, Emma M. Amaro, Ana C. Winter, Jennifer R. Dorn, Jonas F. Elliott, Hunter L. Mchedlishvili, Nunu McClelland, Sarah E. Porter, Iain M. Posch, Markus Toso, Alberto Danuser, Gaudenz McAinsh, Andrew D. Meraldi, Patrick Swedlow, Jason R. J Cell Biol Research Articles During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion. The Rockefeller University Press 2010-03-08 /pmc/articles/PMC2835940/ /pubmed/20212316 http://dx.doi.org/10.1083/jcb.200909005 Text en © 2010 Jaqaman et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Jaqaman, Khuloud King, Emma M. Amaro, Ana C. Winter, Jennifer R. Dorn, Jonas F. Elliott, Hunter L. Mchedlishvili, Nunu McClelland, Sarah E. Porter, Iain M. Posch, Markus Toso, Alberto Danuser, Gaudenz McAinsh, Andrew D. Meraldi, Patrick Swedlow, Jason R. Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
title | Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
title_full | Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
title_fullStr | Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
title_full_unstemmed | Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
title_short | Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
title_sort | kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835940/ https://www.ncbi.nlm.nih.gov/pubmed/20212316 http://dx.doi.org/10.1083/jcb.200909005 |
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