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A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle

The TAR RNA of HIV was engineered as an siRNA delivery vehicle to develop a combinatorial therapeutic approach. The TAR backbone was found to be a versatile backbone for expressing siRNAs. Upon expression in human cells, pronounced and specific inhibition of reporter gene expression was observed wit...

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Detalles Bibliográficos
Autores principales: Unwalla, Hoshang J, Rossi, John J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836314/
https://www.ncbi.nlm.nih.gov/pubmed/20144240
http://dx.doi.org/10.1186/1743-422X-7-33
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author Unwalla, Hoshang J
Rossi, John J
author_facet Unwalla, Hoshang J
Rossi, John J
author_sort Unwalla, Hoshang J
collection PubMed
description The TAR RNA of HIV was engineered as an siRNA delivery vehicle to develop a combinatorial therapeutic approach. The TAR backbone was found to be a versatile backbone for expressing siRNAs. Upon expression in human cells, pronounced and specific inhibition of reporter gene expression was observed with TARmiR. The resulting TARmiR construct retained its ability to bind Tat and mediate RNAi. TARmiR was able to inhibit HIV gene expression as a TAR decoy and by RNA interference when challenged with infectious proviral DNA. The implications of this dual function therapeutic would be discussed.
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spelling pubmed-28363142010-03-11 A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle Unwalla, Hoshang J Rossi, John J Virol J Methodology The TAR RNA of HIV was engineered as an siRNA delivery vehicle to develop a combinatorial therapeutic approach. The TAR backbone was found to be a versatile backbone for expressing siRNAs. Upon expression in human cells, pronounced and specific inhibition of reporter gene expression was observed with TARmiR. The resulting TARmiR construct retained its ability to bind Tat and mediate RNAi. TARmiR was able to inhibit HIV gene expression as a TAR decoy and by RNA interference when challenged with infectious proviral DNA. The implications of this dual function therapeutic would be discussed. BioMed Central 2010-02-10 /pmc/articles/PMC2836314/ /pubmed/20144240 http://dx.doi.org/10.1186/1743-422X-7-33 Text en Copyright ©2010 Unwalla and Rossi; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Unwalla, Hoshang J
Rossi, John J
A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
title A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
title_full A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
title_fullStr A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
title_full_unstemmed A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
title_short A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
title_sort dual function tar decoy serves as an anti-hiv sirna delivery vehicle
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836314/
https://www.ncbi.nlm.nih.gov/pubmed/20144240
http://dx.doi.org/10.1186/1743-422X-7-33
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