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Plasmid Construction Using Recombination Activity in the Fission Yeast Schizosaccharomyces pombe

BACKGROUND: Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligase. Another potent method to construct plasmids,...

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Detalles Bibliográficos
Autores principales: Chino, Ayako, Watanabe, Kenji, Moriya, Hisao
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836380/
https://www.ncbi.nlm.nih.gov/pubmed/20300182
http://dx.doi.org/10.1371/journal.pone.0009652