Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR

BACKGROUND: Linear regression of efficiency or LRE introduced a new paradigm for conducting absolute quantification, which does not require standard curves, can generate absolute accuracies of ±25% and has single molecule sensitivity. Derived from adapting the classic Boltzmann sigmoidal function to...

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Detalles Bibliográficos
Autores principales: Rutledge, Robert G., Stewart, Don
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840021/
https://www.ncbi.nlm.nih.gov/pubmed/20305810
http://dx.doi.org/10.1371/journal.pone.0009731

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840021/
https://www.ncbi.nlm.nih.gov/pubmed/20305810
http://dx.doi.org/10.1371/journal.pone.0009731

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