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Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR
BACKGROUND: Linear regression of efficiency or LRE introduced a new paradigm for conducting absolute quantification, which does not require standard curves, can generate absolute accuracies of ±25% and has single molecule sensitivity. Derived from adapting the classic Boltzmann sigmoidal function to...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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Public Library of Science
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840021/ https://www.ncbi.nlm.nih.gov/pubmed/20305810 http://dx.doi.org/10.1371/journal.pone.0009731 |