Gene-specific FACS sorting method for target selection in high-throughput amplicon sequencing

BACKGROUND: In addition to shotgun sequencing, next generation sequencing has been shown to be suitable for deep sequencing of many specific PCR-amplified target genes in parallel. However, unspecific product formation is a common problem in amplicon sequencing since these fragments are difficult to...

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Detalles Bibliográficos
Autores principales: Sandberg, Julia, Neiman, Marten, Ahmadian, Afshin, Lundeberg, Joakim
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842249/
https://www.ncbi.nlm.nih.gov/pubmed/20184782
http://dx.doi.org/10.1186/1471-2164-11-140

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842249/
https://www.ncbi.nlm.nih.gov/pubmed/20184782
http://dx.doi.org/10.1186/1471-2164-11-140

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