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Gene-specific FACS sorting method for target selection in high-throughput amplicon sequencing
BACKGROUND: In addition to shotgun sequencing, next generation sequencing has been shown to be suitable for deep sequencing of many specific PCR-amplified target genes in parallel. However, unspecific product formation is a common problem in amplicon sequencing since these fragments are difficult to...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842249/ https://www.ncbi.nlm.nih.gov/pubmed/20184782 http://dx.doi.org/10.1186/1471-2164-11-140 |