LC/MS analysis of cellular RNA reveals NAD-linked RNA

We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule, revealing NAD-linked RNA in E. coli and S. venezuelae. Subsequent characterization shows NAD is a 5’ modification of RNA, cannot be installed in vitro through ab...

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Detalles Bibliográficos
Autores principales: Chen, Y. Grace, Kowtoniuk, Walter E., Agarwal, Isha, Shen, Yinghua, Liu, David R.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842606/
https://www.ncbi.nlm.nih.gov/pubmed/19820715
http://dx.doi.org/10.1038/nchembio.235
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author Chen, Y. Grace
Kowtoniuk, Walter E.
Agarwal, Isha
Shen, Yinghua
Liu, David R.
author_facet Chen, Y. Grace
Kowtoniuk, Walter E.
Agarwal, Isha
Shen, Yinghua
Liu, David R.
author_sort Chen, Y. Grace
collection PubMed
description We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule, revealing NAD-linked RNA in E. coli and S. venezuelae. Subsequent characterization shows NAD is a 5’ modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs, and is present at a surprisingly high abundance of ~3000 copies per cell.
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spelling pubmed-28426062010-06-01 LC/MS analysis of cellular RNA reveals NAD-linked RNA Chen, Y. Grace Kowtoniuk, Walter E. Agarwal, Isha Shen, Yinghua Liu, David R. Nat Chem Biol Article We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule, revealing NAD-linked RNA in E. coli and S. venezuelae. Subsequent characterization shows NAD is a 5’ modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs, and is present at a surprisingly high abundance of ~3000 copies per cell. 2009-10-11 2009-12 /pmc/articles/PMC2842606/ /pubmed/19820715 http://dx.doi.org/10.1038/nchembio.235 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chen, Y. Grace
Kowtoniuk, Walter E.
Agarwal, Isha
Shen, Yinghua
Liu, David R.
LC/MS analysis of cellular RNA reveals NAD-linked RNA
title LC/MS analysis of cellular RNA reveals NAD-linked RNA
title_full LC/MS analysis of cellular RNA reveals NAD-linked RNA
title_fullStr LC/MS analysis of cellular RNA reveals NAD-linked RNA
title_full_unstemmed LC/MS analysis of cellular RNA reveals NAD-linked RNA
title_short LC/MS analysis of cellular RNA reveals NAD-linked RNA
title_sort lc/ms analysis of cellular rna reveals nad-linked rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2842606/
https://www.ncbi.nlm.nih.gov/pubmed/19820715
http://dx.doi.org/10.1038/nchembio.235
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