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Evaluation of cost-effective total nucleic acids extraction protocols for cultured Mycobacterium tuberculosis; a comparison by PCR amplification of genes associated with drug resistance

BACKGROUND: The emergence of drug resistant strains of Mycobacterium tuberculosis complex has made the management of tuberculosis difficult. Also, Mycobacterium species has a peculiar cell wall, made of an impermeable complex structure rich in mycolate, making the lyses of its cell difficult. In ord...

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Detalles Bibliográficos
Autores principales: Awua, Adolf K, Doe, Edna D, Gyamfi, Oti K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845596/
https://www.ncbi.nlm.nih.gov/pubmed/20187921
http://dx.doi.org/10.1186/1756-0500-3-48