RNA isolation method for single embryo transcriptome analysis in zebrafish

BACKGROUND: Transcriptome analysis during embryogenesis usually requires pooling of embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent the average mRNA levels of pooled samples and the biological variation among individuals is confounded. This can irreversibly...

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Detalles Bibliográficos
Autores principales: de Jong, Mark, Rauwerda, Han, Bruning, Oskar, Verkooijen, Jurgo, Spaink, Herman P, Breit, Timo M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845602/
https://www.ncbi.nlm.nih.gov/pubmed/20233395
http://dx.doi.org/10.1186/1756-0500-3-73

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845602/
https://www.ncbi.nlm.nih.gov/pubmed/20233395
http://dx.doi.org/10.1186/1756-0500-3-73

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