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Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability

Signaling proteins often control multiple aspects of cell morphogenesis. Yet the mechanisms that govern their pleiotropic behavior are often unclear. Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in...

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Detalles Bibliográficos
Autores principales: Rallis, Andrew, Moore, Coralie, Ng, Julian
Formato: Texto
Lenguaje:English
Publicado: Elsevier 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845820/
https://www.ncbi.nlm.nih.gov/pubmed/20035736
http://dx.doi.org/10.1016/j.ydbio.2009.12.016
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author Rallis, Andrew
Moore, Coralie
Ng, Julian
author_facet Rallis, Andrew
Moore, Coralie
Ng, Julian
author_sort Rallis, Andrew
collection PubMed
description Signaling proteins often control multiple aspects of cell morphogenesis. Yet the mechanisms that govern their pleiotropic behavior are often unclear. Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in Drosophila mushroom body (MB) neurons. In the complete absence of Drosophila JNK (Basket), MB axons fail to stabilize, leading to their subsequent degeneration. However, with a partial loss of Basket (Bsk), or of one of the upstream JNK kinases, Hemipterous or Mkk4, these axons overextend. This suggests that Bsk activity prevents axons from destabilizing, resulting in degeneration and overextension beyond their terminal targets. These distinct phenotypes require different threshold activities involving the convergent action of two distinct JNK kinases. We show that sustained Bsk signals are essential throughout development and act additively but are dispensable at adulthood. We also suggest that graded Bsk inputs are translated into AP-1 transcriptional outputs consisting of Fos and Jun proteins.
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spelling pubmed-28458202010-03-31 Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability Rallis, Andrew Moore, Coralie Ng, Julian Dev Biol Article Signaling proteins often control multiple aspects of cell morphogenesis. Yet the mechanisms that govern their pleiotropic behavior are often unclear. Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in Drosophila mushroom body (MB) neurons. In the complete absence of Drosophila JNK (Basket), MB axons fail to stabilize, leading to their subsequent degeneration. However, with a partial loss of Basket (Bsk), or of one of the upstream JNK kinases, Hemipterous or Mkk4, these axons overextend. This suggests that Bsk activity prevents axons from destabilizing, resulting in degeneration and overextension beyond their terminal targets. These distinct phenotypes require different threshold activities involving the convergent action of two distinct JNK kinases. We show that sustained Bsk signals are essential throughout development and act additively but are dispensable at adulthood. We also suggest that graded Bsk inputs are translated into AP-1 transcriptional outputs consisting of Fos and Jun proteins. Elsevier 2010-03-01 /pmc/articles/PMC2845820/ /pubmed/20035736 http://dx.doi.org/10.1016/j.ydbio.2009.12.016 Text en © 2010 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Rallis, Andrew
Moore, Coralie
Ng, Julian
Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
title Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
title_full Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
title_fullStr Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
title_full_unstemmed Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
title_short Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
title_sort signal strength and signal duration define two distinct aspects of jnk-regulated axon stability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845820/
https://www.ncbi.nlm.nih.gov/pubmed/20035736
http://dx.doi.org/10.1016/j.ydbio.2009.12.016
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AT ngjulian signalstrengthandsignaldurationdefinetwodistinctaspectsofjnkregulatedaxonstability