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Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability
Signaling proteins often control multiple aspects of cell morphogenesis. Yet the mechanisms that govern their pleiotropic behavior are often unclear. Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Elsevier
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845820/ https://www.ncbi.nlm.nih.gov/pubmed/20035736 http://dx.doi.org/10.1016/j.ydbio.2009.12.016 |
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author | Rallis, Andrew Moore, Coralie Ng, Julian |
author_facet | Rallis, Andrew Moore, Coralie Ng, Julian |
author_sort | Rallis, Andrew |
collection | PubMed |
description | Signaling proteins often control multiple aspects of cell morphogenesis. Yet the mechanisms that govern their pleiotropic behavior are often unclear. Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in Drosophila mushroom body (MB) neurons. In the complete absence of Drosophila JNK (Basket), MB axons fail to stabilize, leading to their subsequent degeneration. However, with a partial loss of Basket (Bsk), or of one of the upstream JNK kinases, Hemipterous or Mkk4, these axons overextend. This suggests that Bsk activity prevents axons from destabilizing, resulting in degeneration and overextension beyond their terminal targets. These distinct phenotypes require different threshold activities involving the convergent action of two distinct JNK kinases. We show that sustained Bsk signals are essential throughout development and act additively but are dispensable at adulthood. We also suggest that graded Bsk inputs are translated into AP-1 transcriptional outputs consisting of Fos and Jun proteins. |
format | Text |
id | pubmed-2845820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-28458202010-03-31 Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability Rallis, Andrew Moore, Coralie Ng, Julian Dev Biol Article Signaling proteins often control multiple aspects of cell morphogenesis. Yet the mechanisms that govern their pleiotropic behavior are often unclear. Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in Drosophila mushroom body (MB) neurons. In the complete absence of Drosophila JNK (Basket), MB axons fail to stabilize, leading to their subsequent degeneration. However, with a partial loss of Basket (Bsk), or of one of the upstream JNK kinases, Hemipterous or Mkk4, these axons overextend. This suggests that Bsk activity prevents axons from destabilizing, resulting in degeneration and overextension beyond their terminal targets. These distinct phenotypes require different threshold activities involving the convergent action of two distinct JNK kinases. We show that sustained Bsk signals are essential throughout development and act additively but are dispensable at adulthood. We also suggest that graded Bsk inputs are translated into AP-1 transcriptional outputs consisting of Fos and Jun proteins. Elsevier 2010-03-01 /pmc/articles/PMC2845820/ /pubmed/20035736 http://dx.doi.org/10.1016/j.ydbio.2009.12.016 Text en © 2010 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Rallis, Andrew Moore, Coralie Ng, Julian Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability |
title | Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability |
title_full | Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability |
title_fullStr | Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability |
title_full_unstemmed | Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability |
title_short | Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability |
title_sort | signal strength and signal duration define two distinct aspects of jnk-regulated axon stability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845820/ https://www.ncbi.nlm.nih.gov/pubmed/20035736 http://dx.doi.org/10.1016/j.ydbio.2009.12.016 |
work_keys_str_mv | AT rallisandrew signalstrengthandsignaldurationdefinetwodistinctaspectsofjnkregulatedaxonstability AT moorecoralie signalstrengthandsignaldurationdefinetwodistinctaspectsofjnkregulatedaxonstability AT ngjulian signalstrengthandsignaldurationdefinetwodistinctaspectsofjnkregulatedaxonstability |