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Dapdiamides, Tripeptide Antibiotics Formed by Unconventional Amide Ligases†

[Image: see text] Construction of a genomic DNA library from Pantoea agglomerans strain CU0119 and screening against the plant pathogen Erwinia amylovora yielded a new family of antibiotics, dapdiamides A−E (1−5). The structures were established through 2D-NMR experiments and mass spectrometry, as w...

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Detalles Bibliográficos
Autores principales: Dawlaty, Jessica, Zhang, Xiaorong, Fischbach, Michael A., Clardy, Jon
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society and American Society of Pharmacognosy 2009
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846032/
https://www.ncbi.nlm.nih.gov/pubmed/20041689
http://dx.doi.org/10.1021/np900685z
Descripción
Sumario:[Image: see text] Construction of a genomic DNA library from Pantoea agglomerans strain CU0119 and screening against the plant pathogen Erwinia amylovora yielded a new family of antibiotics, dapdiamides A−E (1−5). The structures were established through 2D-NMR experiments and mass spectrometry, as well as the synthesis of dapdiamide A (1). Transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. The dapdiamide family’s promiscuous biosynthetic pathway contains two unconventional amide ligases that are predicted to couple its constituent monomers.