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Specific Knockdown of OCT4 in Human Embryonic Stem Cells by Inducible Short Hairpin RNA Interference

Manipulation of gene function in embryonic stem cells by either over expression or downregulation is critical for understanding their subsequent cell fate. We have developed a tetracycline-inducible short hairpin RNA interference (shRNAi) for human embryonic stem cells (hESCs) and demonstrated doxyc...

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Detalles Bibliográficos
Autores principales: Zafarana, Gaetano, Avery, Stuart R, Avery, Katie, Moore, Harry D, Andrews, Peter W
Formato: Texto
Lenguaje:English
Publicado: Wiley Subscription Services, Inc., A Wiley Company 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847189/
https://www.ncbi.nlm.nih.gov/pubmed/19350677
http://dx.doi.org/10.1002/stem.5