Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA

DNA sequences determined from ancient organisms have high error rates, primarily due to uracil bases created by cytosine deamination. We use synthetic oligonucleotides, as well as DNA extracted from mammoth and Neandertal remains, to show that treatment with uracil–DNA–glycosylase and endonuclease V...

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Detalles Bibliográficos
Autores principales: Briggs, Adrian W., Stenzel, Udo, Meyer, Matthias, Krause, Johannes, Kircher, Martin, Pääbo, Svante
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847228/
https://www.ncbi.nlm.nih.gov/pubmed/20028723
http://dx.doi.org/10.1093/nar/gkp1163

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847228/
https://www.ncbi.nlm.nih.gov/pubmed/20028723
http://dx.doi.org/10.1093/nar/gkp1163

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