Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli

Despite recent advances in sequencing, complete finishing of large genomes and analysis of novel proteins they encode typically require cloning of specific regions. However, many of these fragments are extremely difficult to clone in current vectors. Superhelical stress in circular plasmids can gene...

Descripción completa

Detalles Bibliográficos
Autores principales: Godiska, Ronald, Mead, David, Dhodda, Vinay, Wu, Chengcang, Hochstein, Rebecca, Karsi, Attila, Usdin, Karen, Entezam, Ali, Ravin, Nikolai
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847241/
https://www.ncbi.nlm.nih.gov/pubmed/20040575
http://dx.doi.org/10.1093/nar/gkp1181

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847241/
https://www.ncbi.nlm.nih.gov/pubmed/20040575
http://dx.doi.org/10.1093/nar/gkp1181

Ejemplares similares