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Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli
Despite recent advances in sequencing, complete finishing of large genomes and analysis of novel proteins they encode typically require cloning of specific regions. However, many of these fragments are extremely difficult to clone in current vectors. Superhelical stress in circular plasmids can gene...
| Autores principales: | , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847241/ https://www.ncbi.nlm.nih.gov/pubmed/20040575 http://dx.doi.org/10.1093/nar/gkp1181 |