Cargando…

PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture

To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain...

Descripción completa

Detalles Bibliográficos
Autores principales: Kurasawa, Yasuhiro, Yu-Lee, Li-yuan
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847523/
https://www.ncbi.nlm.nih.gov/pubmed/20130082
http://dx.doi.org/10.1091/mbc.E09-11-0950
_version_ 1782179571827736576
author Kurasawa, Yasuhiro
Yu-Lee, Li-yuan
author_facet Kurasawa, Yasuhiro
Yu-Lee, Li-yuan
author_sort Kurasawa, Yasuhiro
collection PubMed
description To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain chromosome architecture during prometaphase. PICH knockdown results in a loss of Plk1 from the chromosome arm and an increase in highly disorganized “wavy” chromosomes that exhibit an “open” or “X-shaped” configuration, consistent with a loss of chromosome arm cohesion. Such chromosome disorganization occurs with essentially no change in the localization of condensin or cohesin on chromosomes. Interestingly, the chromosome disorganization could be prevented by treatment with a topoisomerase II inhibitor ICRF-193, suggesting that the PICH–Plk1 complex normally maintains chromosome architecture in a manner that involves topoisomerase II activity. PICH knockdown does not affect initial chromosome compaction at prophase but causes anaphase DNA bridge formation and failed abscission. Our studies suggest that the PICH–Plk1 complex plays a critical role in maintaining prometaphase chromosome architecture.
format Text
id pubmed-2847523
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-28475232010-06-16 PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture Kurasawa, Yasuhiro Yu-Lee, Li-yuan Mol Biol Cell Articles To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain chromosome architecture during prometaphase. PICH knockdown results in a loss of Plk1 from the chromosome arm and an increase in highly disorganized “wavy” chromosomes that exhibit an “open” or “X-shaped” configuration, consistent with a loss of chromosome arm cohesion. Such chromosome disorganization occurs with essentially no change in the localization of condensin or cohesin on chromosomes. Interestingly, the chromosome disorganization could be prevented by treatment with a topoisomerase II inhibitor ICRF-193, suggesting that the PICH–Plk1 complex normally maintains chromosome architecture in a manner that involves topoisomerase II activity. PICH knockdown does not affect initial chromosome compaction at prophase but causes anaphase DNA bridge formation and failed abscission. Our studies suggest that the PICH–Plk1 complex plays a critical role in maintaining prometaphase chromosome architecture. The American Society for Cell Biology 2010-04-01 /pmc/articles/PMC2847523/ /pubmed/20130082 http://dx.doi.org/10.1091/mbc.E09-11-0950 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Kurasawa, Yasuhiro
Yu-Lee, Li-yuan
PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
title PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
title_full PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
title_fullStr PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
title_full_unstemmed PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
title_short PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
title_sort pich and cotargeted plk1 coordinately maintain prometaphase chromosome arm architecture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847523/
https://www.ncbi.nlm.nih.gov/pubmed/20130082
http://dx.doi.org/10.1091/mbc.E09-11-0950
work_keys_str_mv AT kurasawayasuhiro pichandcotargetedplk1coordinatelymaintainprometaphasechromosomearmarchitecture
AT yuleeliyuan pichandcotargetedplk1coordinatelymaintainprometaphasechromosomearmarchitecture