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PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture
To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847523/ https://www.ncbi.nlm.nih.gov/pubmed/20130082 http://dx.doi.org/10.1091/mbc.E09-11-0950 |
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author | Kurasawa, Yasuhiro Yu-Lee, Li-yuan |
author_facet | Kurasawa, Yasuhiro Yu-Lee, Li-yuan |
author_sort | Kurasawa, Yasuhiro |
collection | PubMed |
description | To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain chromosome architecture during prometaphase. PICH knockdown results in a loss of Plk1 from the chromosome arm and an increase in highly disorganized “wavy” chromosomes that exhibit an “open” or “X-shaped” configuration, consistent with a loss of chromosome arm cohesion. Such chromosome disorganization occurs with essentially no change in the localization of condensin or cohesin on chromosomes. Interestingly, the chromosome disorganization could be prevented by treatment with a topoisomerase II inhibitor ICRF-193, suggesting that the PICH–Plk1 complex normally maintains chromosome architecture in a manner that involves topoisomerase II activity. PICH knockdown does not affect initial chromosome compaction at prophase but causes anaphase DNA bridge formation and failed abscission. Our studies suggest that the PICH–Plk1 complex plays a critical role in maintaining prometaphase chromosome architecture. |
format | Text |
id | pubmed-2847523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28475232010-06-16 PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture Kurasawa, Yasuhiro Yu-Lee, Li-yuan Mol Biol Cell Articles To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain chromosome architecture during prometaphase. PICH knockdown results in a loss of Plk1 from the chromosome arm and an increase in highly disorganized “wavy” chromosomes that exhibit an “open” or “X-shaped” configuration, consistent with a loss of chromosome arm cohesion. Such chromosome disorganization occurs with essentially no change in the localization of condensin or cohesin on chromosomes. Interestingly, the chromosome disorganization could be prevented by treatment with a topoisomerase II inhibitor ICRF-193, suggesting that the PICH–Plk1 complex normally maintains chromosome architecture in a manner that involves topoisomerase II activity. PICH knockdown does not affect initial chromosome compaction at prophase but causes anaphase DNA bridge formation and failed abscission. Our studies suggest that the PICH–Plk1 complex plays a critical role in maintaining prometaphase chromosome architecture. The American Society for Cell Biology 2010-04-01 /pmc/articles/PMC2847523/ /pubmed/20130082 http://dx.doi.org/10.1091/mbc.E09-11-0950 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Kurasawa, Yasuhiro Yu-Lee, Li-yuan PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture |
title | PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture |
title_full | PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture |
title_fullStr | PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture |
title_full_unstemmed | PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture |
title_short | PICH and Cotargeted Plk1 Coordinately Maintain Prometaphase Chromosome Arm Architecture |
title_sort | pich and cotargeted plk1 coordinately maintain prometaphase chromosome arm architecture |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847523/ https://www.ncbi.nlm.nih.gov/pubmed/20130082 http://dx.doi.org/10.1091/mbc.E09-11-0950 |
work_keys_str_mv | AT kurasawayasuhiro pichandcotargetedplk1coordinatelymaintainprometaphasechromosomearmarchitecture AT yuleeliyuan pichandcotargetedplk1coordinatelymaintainprometaphasechromosomearmarchitecture |