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Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data

We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantific...

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Detalles Bibliográficos
Autores principales: Marullo, Manuela, Zuccato, Chiara, Mariotti, Caterina, Lahiri, Nayana, Tabrizi, Sarah J, Di Donato, Stefano, Cattaneo, Elena
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847721/
https://www.ncbi.nlm.nih.gov/pubmed/20109193
http://dx.doi.org/10.1186/gb-2010-11-1-r9