Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data

We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantific...

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Detalles Bibliográficos
Autores principales: Marullo, Manuela, Zuccato, Chiara, Mariotti, Caterina, Lahiri, Nayana, Tabrizi, Sarah J, Di Donato, Stefano, Cattaneo, Elena
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847721/
https://www.ncbi.nlm.nih.gov/pubmed/20109193
http://dx.doi.org/10.1186/gb-2010-11-1-r9
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author Marullo, Manuela
Zuccato, Chiara
Mariotti, Caterina
Lahiri, Nayana
Tabrizi, Sarah J
Di Donato, Stefano
Cattaneo, Elena
author_facet Marullo, Manuela
Zuccato, Chiara
Mariotti, Caterina
Lahiri, Nayana
Tabrizi, Sarah J
Di Donato, Stefano
Cattaneo, Elena
author_sort Marullo, Manuela
collection PubMed
description We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This result is particularly important for clinical diagnosis and biomarker validation studies based on mRNA detection in human blood.
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spelling pubmed-28477212010-03-31 Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data Marullo, Manuela Zuccato, Chiara Mariotti, Caterina Lahiri, Nayana Tabrizi, Sarah J Di Donato, Stefano Cattaneo, Elena Genome Biol Method We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This result is particularly important for clinical diagnosis and biomarker validation studies based on mRNA detection in human blood. BioMed Central 2010 2010-01-28 /pmc/articles/PMC2847721/ /pubmed/20109193 http://dx.doi.org/10.1186/gb-2010-11-1-r9 Text en Copyright ©2010 Marullo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Marullo, Manuela
Zuccato, Chiara
Mariotti, Caterina
Lahiri, Nayana
Tabrizi, Sarah J
Di Donato, Stefano
Cattaneo, Elena
Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
title Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
title_full Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
title_fullStr Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
title_full_unstemmed Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
title_short Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
title_sort expressed alu repeats as a novel, reliable tool for normalization of real-time quantitative rt-pcr data
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847721/
https://www.ncbi.nlm.nih.gov/pubmed/20109193
http://dx.doi.org/10.1186/gb-2010-11-1-r9
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