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Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantific...
Autores principales: | Marullo, Manuela, Zuccato, Chiara, Mariotti, Caterina, Lahiri, Nayana, Tabrizi, Sarah J, Di Donato, Stefano, Cattaneo, Elena |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847721/ https://www.ncbi.nlm.nih.gov/pubmed/20109193 http://dx.doi.org/10.1186/gb-2010-11-1-r9 |
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