Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design

BACKGROUND: RNA interference (RNAi) has been one of the most rapidly expanding areas of biological research in the past decade, revolutionizing the ability to analyze gene function. Thorough validation of siRNA duplexes is required prior to use in experimental systems, ideally by western blotting to...

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Detalles Bibliográficos
Autores principales: Holmes, Katherine, Williams, Catrin M, Chapman, Elinor A, Cross, Michael J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850347/
https://www.ncbi.nlm.nih.gov/pubmed/20199660
http://dx.doi.org/10.1186/1756-0500-3-53

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850347/
https://www.ncbi.nlm.nih.gov/pubmed/20199660
http://dx.doi.org/10.1186/1756-0500-3-53

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