In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

BACKGROUND: Multicellular tumour sphere models have been shown to closely mimic phenotype characteristics of in vivo solid tumours, or to allow in vitro propagation of cancer stem cells (CSCs). CSCs are usually characterized by the expression of specific membrane markers using flow cytometry (FC) af...

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Detalles Bibliográficos
Autores principales: Weiswald, Louis-Bastien, Guinebretière, Jean-Marc, Richon, Sophie, Bellet, Dominique, Saubaméa, Bruno, Dangles-Marie, Virginie
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851689/
https://www.ncbi.nlm.nih.gov/pubmed/20307308
http://dx.doi.org/10.1186/1471-2407-10-106

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851689/
https://www.ncbi.nlm.nih.gov/pubmed/20307308
http://dx.doi.org/10.1186/1471-2407-10-106

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