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Single Vector System for Efficient N-myristoylation of Recombinant Proteins in E. coli

BACKGROUND: N-myristoylation is a crucial covalent modification of numerous eukaryotic and viral proteins that is catalyzed by N-myristoyltransferase (NMT). Prokaryotes are lacking endogeneous NMT activity. Recombinant production of N-myristoylated proteins in E. coli cells can be achieved by coexpr...

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Detalles Bibliográficos
Autores principales: Glück, Julian M., Hoffmann, Silke, Koenig, Bernd W., Willbold, Dieter
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852408/
https://www.ncbi.nlm.nih.gov/pubmed/20404920
http://dx.doi.org/10.1371/journal.pone.0010081