Cargando…

Single Vector System for Efficient N-myristoylation of Recombinant Proteins in E. coli

BACKGROUND: N-myristoylation is a crucial covalent modification of numerous eukaryotic and viral proteins that is catalyzed by N-myristoyltransferase (NMT). Prokaryotes are lacking endogeneous NMT activity. Recombinant production of N-myristoylated proteins in E. coli cells can be achieved by coexpr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Glück, Julian M., Hoffmann, Silke, Koenig, Bernd W., Willbold, Dieter
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852408/ https://www.ncbi.nlm.nih.gov/pubmed/20404920 http://dx.doi.org/10.1371/journal.pone.0010081

Ejemplares similares

N‐Lauroylation during the Expression of Recombinant N‐Myristoylated Proteins: Implications and Solutions
por: Flamm, Andrea Gabriele, et al.
Publicado: (2015)

Recombinant Production of the Amino Terminal Cytoplasmic Region of Dengue Virus Non-Structural Protein 4A for Structural Studies
por: Hung, Yu-Fu, et al.
Publicado: (2014)

Data on the identity and myristoylation state of recombinant, purified hippocalcin
por: Krishnan, Anuradha, et al.
Publicado: (2016)

Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration
por: Broncel, Malgorzata, et al.
Publicado: (2020)

Prediction of N-myristoylation modification of proteins by SVM
por: Cao, Wei, et al.
Publicado: (2011)

Efficient generation of double heterologous promoter controlled oncolytic adenovirus vectors by a single homologous recombination step in Escherichia coli
por: Hoffmann, Dennis, et al.
Publicado: (2006)

Predicting N-terminal myristoylation sites in plant proteins
por: Podell, Sheila, et al.
Publicado: (2004)

Targeting N-myristoylation for therapy of B-cell lymphomas
por: Beauchamp, Erwan, et al.
Publicado: (2020)

N-myristoylation: from cell biology to translational medicine
por: Yuan, Meng, et al.
Publicado: (2020)

Myristoylation versus SRP
por: LeBrasseur, Nicole
Publicado: (2005)

Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes
por: Hung, Yu-Fu, et al.
Publicado: (2015)

Data on the calcium-induced mobility shift of myristoylated and non-myristoylated forms of neurocalcin delta
por: Viviano, Jeffrey, et al.
Publicado: (2016)

Protein N-myristoylation: functions and mechanisms in control of innate immunity
por: Wang, Bin, et al.
Publicado: (2021)

Application of Liquid-Liquid Extraction for N-terminal Myristoylation Proteomics
por: Tsumagari, Kazuya, et al.
Publicado: (2023)

MYRbase: analysis of genome-wide glycine myristoylation enlarges the functional spectrum of eukaryotic myristoylated proteins
por: Maurer-Stroh, Sebastian, et al.
Publicado: (2004)

Design and Characterization of Myristoylated and Non-Myristoylated Peptides Effective against Candida spp. Clinical Isolates
por: Bugli, Francesca, et al.
Publicado: (2022)

Myristoylation profiling in human cells and zebrafish
por: Broncel, Malgorzata, et al.
Publicado: (2015)

Protein Lipidation by Palmitoylation and Myristoylation in Cancer
por: Fhu, Chee Wai, et al.
Publicado: (2021)

Global profiling of co- and post-translationally N-myristoylated proteomes in human cells
por: Thinon, Emmanuelle, et al.
Publicado: (2014)

Cellular FRET-Biosensors to Detect Membrane Targeting Inhibitors of N-Myristoylated Proteins
por: Najumudeen, Arafath Kaja, et al.
Publicado: (2013)

Crystal structure of the N-myristoylated lipopeptide-bound MHC class I complex
por: Morita, Daisuke, et al.
Publicado: (2016)

Targeting protein myristoylation for the treatment of prostate cancer
por: Sulejmani, Essilvo, et al.
Publicado: (2018)

Myristoylation: An Important Protein Modification in the Immune Response
por: Udenwobele, Daniel Ikenna, et al.
Publicado: (2017)

A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coli
por: Banerjee, Sampali, et al.
Publicado: (2010)

Correction: Cellular FRET-Biosensors to Detect Membrane Targeting Inhibitors of N-Myristoylated Proteins
por: Najumudeen, Arafath Kaja, et al.
Publicado: (2014)

Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry
por: Boyle, Patrick C., et al.
Publicado: (2016)

Mouse Stbd1 is N-myristoylated and affects ER–mitochondria association and mitochondrial morphology
por: Demetriadou, Anthi, et al.
Publicado: (2017)

N-terminal Myristoylation Enhanced the Antimicrobial Activity of Antimicrobial Peptide PMAP-36PW
por: Liu, Yongqing, et al.
Publicado: (2020)

Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress and invasion
por: Schlott, Anja C., et al.
Publicado: (2021)

Potential role of HIV-1 Nef and human M6B in HIV-associated neurological disorders
por: Moetter, Jessica, et al.
Publicado: (2010)

Encapsulating Cas9 into extracellular vesicles by protein myristoylation
por: Whitley, Joseph Andrew, et al.
Publicado: (2022)

Myristoyl-CoA:protein N-myristoyltransferase depletion in trypanosomes causes avirulence and endocytic defects
por: Price, Helen P., et al.
Publicado: (2010)

Myristic acid auxotrophy caused by mutation of S. cerevisiae myristoyl- CoA:protein N-myristoyltransferase
Publicado: (1991)

Biochemical Characterization of Bovine Brain Myristoyl-CoA:Protein N-Myristoyltransferase Type 2
por: Selvakumar, Ponniah, et al.
Publicado: (2009)

An N-Myristoylated Globin with a Redox-Sensing Function That Regulates the Defecation Cycle in Caenorhabditis elegans
por: Tilleman, Lesley, et al.
Publicado: (2012)

A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
por: Jang, Chuan-Wei, et al.
Publicado: (2013)

Efficient recovery of recombinant CRM197 expressed as inclusion bodies in E.coli
por: Park, Ah-Reum, et al.
Publicado: (2018)

Conformational regulation and target-myristoyl switch of calcineurin B homologous protein 3
por: Becker, Florian, et al.
Publicado: (2023)

Immobilization of Homogeneous Monomeric, Oligomeric and Fibrillar Aβ Species for Reliable SPR Measurements
por: Frenzel, Daniel, et al.
Publicado: (2014)

The residue at position 5 of the N-terminal region of Src and Fyn modulates their myristoylation, palmitoylation, and membrane interactions
por: Gottlieb-Abraham, Efrat, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_397b56noghek4t1gejmob32vt8