A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site

Metal-dependent nucleases that generate double-strand breaks in DNA often possess two symmetrically-equivalent subunits, arranged so that the active sites from each subunit act on opposite DNA strands. Restriction endonuclease BfiI belongs to the phospholipase D (PLD) superfamily and does not requir...

Descripción completa

Detalles Bibliográficos
Autores principales: Sasnauskas, Giedrius, Zakrys, Linas, Zaremba, Mindaugas, Cosstick, Richard, Gaynor, James W., Halford, Stephen E., Siksnys, Virginijus
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2853115/
https://www.ncbi.nlm.nih.gov/pubmed/20047964
http://dx.doi.org/10.1093/nar/gkp1194

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2853115/
https://www.ncbi.nlm.nih.gov/pubmed/20047964
http://dx.doi.org/10.1093/nar/gkp1194

Ejemplares similares