Glycosylases and AP-cleaving enzymes as a general tool for probe-directed cleavage of ssDNA targets

The current arsenal of molecular tools for site-directed cleavage of single-stranded DNA (ssDNA) is limited. Here, we describe a method for targeted DNA cleavage that requires only the presence of an A nucleotide at the target position. The procedure involves hybridization of a complementary oligonu...

Descripción completa

Detalles Bibliográficos
Autores principales: Howell, W. Mathias, Grundberg, Ida, Faryna, Marta, Landegren, Ulf, Nilsson, Mats
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2853139/
https://www.ncbi.nlm.nih.gov/pubmed/20081204
http://dx.doi.org/10.1093/nar/gkp1238

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2853139/
https://www.ncbi.nlm.nih.gov/pubmed/20081204
http://dx.doi.org/10.1093/nar/gkp1238

Ejemplares similares