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Glycosylases and AP-cleaving enzymes as a general tool for probe-directed cleavage of ssDNA targets
The current arsenal of molecular tools for site-directed cleavage of single-stranded DNA (ssDNA) is limited. Here, we describe a method for targeted DNA cleavage that requires only the presence of an A nucleotide at the target position. The procedure involves hybridization of a complementary oligonu...
Autores principales: | Howell, W. Mathias, Grundberg, Ida, Faryna, Marta, Landegren, Ulf, Nilsson, Mats |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2853139/ https://www.ncbi.nlm.nih.gov/pubmed/20081204 http://dx.doi.org/10.1093/nar/gkp1238 |
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