The optimization of in vitro high-throughput chemical lysis of Escherichia coli. Application to ACP domain of the polyketide synthase ppsC from Mycobacterium tuberculosis

Protein production in Escherichia coli involves high-level expression in a culture, followed by harvesting of the cells and finally their disruption, or lysis, to release the expressed proteins. We compare three high-throughput chemical lysis methods to sonication, using a robotic platform and metho...

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Detalles Bibliográficos
Autores principales: Listwan, Pawel, Pédelacq, Jean-Denis, Lockard, Meghan, Bell, Carolyn, Terwilliger, Thomas C., Waldo, Geoffrey S.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855807/
https://www.ncbi.nlm.nih.gov/pubmed/20069378
http://dx.doi.org/10.1007/s10969-009-9077-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855807/
https://www.ncbi.nlm.nih.gov/pubmed/20069378
http://dx.doi.org/10.1007/s10969-009-9077-8

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