Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain

Multi-polypeptide proteins such as antibodies are difficult to express in prokaryotic systems such as E. coli due to the complexity of protein folding plus secretion. Thus far, proprietary strains or fermenter cultures have been required for appreciable yields. Previous studies have shown that expre...

Descripción completa

Detalles Bibliográficos
Autores principales: Chan, Conrad En Zuo, Lim, Angeline Pei Chiew, Chan, Annie Hoi Yi, MacAry, Paul A., Hanson, Brendon John
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857744/
https://www.ncbi.nlm.nih.gov/pubmed/20422027
http://dx.doi.org/10.1371/journal.pone.0010261

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857744/
https://www.ncbi.nlm.nih.gov/pubmed/20422027
http://dx.doi.org/10.1371/journal.pone.0010261

Ejemplares similares