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Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization
The spatial organization of the genome in the nucleus plays a role in the regulation of gene expression. Whether co-regulated genes are subject to coordinated repositioning to a shared nuclear space is a matter of considerable interest and debate. We investigated the nuclear organization of estrogen...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858706/ https://www.ncbi.nlm.nih.gov/pubmed/20421946 http://dx.doi.org/10.1371/journal.pgen.1000922 |
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author | Kocanova, Silvia Kerr, Elizabeth A. Rafique, Sehrish Boyle, Shelagh Katz, Elad Caze-Subra, Stephanie Bickmore, Wendy A. Bystricky, Kerstin |
author_facet | Kocanova, Silvia Kerr, Elizabeth A. Rafique, Sehrish Boyle, Shelagh Katz, Elad Caze-Subra, Stephanie Bickmore, Wendy A. Bystricky, Kerstin |
author_sort | Kocanova, Silvia |
collection | PubMed |
description | The spatial organization of the genome in the nucleus plays a role in the regulation of gene expression. Whether co-regulated genes are subject to coordinated repositioning to a shared nuclear space is a matter of considerable interest and debate. We investigated the nuclear organization of estrogen receptor alpha (ERα) target genes in human breast epithelial and cancer cell lines, before and after transcriptional activation induced with estradiol. We find that, contrary to another report, the ERα target genes TFF1 and GREB1 are distributed in the nucleoplasm with no particular relationship to each other. The nuclear separation between these genes, as well as between the ERα target genes PGR and CTSD, was unchanged by hormone addition and transcriptional activation with no evidence for co-localization between alleles. Similarly, while the volume occupied by the chromosomes increased, the relative nuclear position of the respective chromosome territories was unaffected by hormone addition. Our results demonstrate that estradiol-induced ERα target genes are not required to co-localize in the nucleus. |
format | Text |
id | pubmed-2858706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28587062010-04-26 Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization Kocanova, Silvia Kerr, Elizabeth A. Rafique, Sehrish Boyle, Shelagh Katz, Elad Caze-Subra, Stephanie Bickmore, Wendy A. Bystricky, Kerstin PLoS Genet Research Article The spatial organization of the genome in the nucleus plays a role in the regulation of gene expression. Whether co-regulated genes are subject to coordinated repositioning to a shared nuclear space is a matter of considerable interest and debate. We investigated the nuclear organization of estrogen receptor alpha (ERα) target genes in human breast epithelial and cancer cell lines, before and after transcriptional activation induced with estradiol. We find that, contrary to another report, the ERα target genes TFF1 and GREB1 are distributed in the nucleoplasm with no particular relationship to each other. The nuclear separation between these genes, as well as between the ERα target genes PGR and CTSD, was unchanged by hormone addition and transcriptional activation with no evidence for co-localization between alleles. Similarly, while the volume occupied by the chromosomes increased, the relative nuclear position of the respective chromosome territories was unaffected by hormone addition. Our results demonstrate that estradiol-induced ERα target genes are not required to co-localize in the nucleus. Public Library of Science 2010-04-22 /pmc/articles/PMC2858706/ /pubmed/20421946 http://dx.doi.org/10.1371/journal.pgen.1000922 Text en Kocanova et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kocanova, Silvia Kerr, Elizabeth A. Rafique, Sehrish Boyle, Shelagh Katz, Elad Caze-Subra, Stephanie Bickmore, Wendy A. Bystricky, Kerstin Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization |
title | Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization |
title_full | Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization |
title_fullStr | Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization |
title_full_unstemmed | Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization |
title_short | Activation of Estrogen-Responsive Genes Does Not Require Their Nuclear Co-Localization |
title_sort | activation of estrogen-responsive genes does not require their nuclear co-localization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858706/ https://www.ncbi.nlm.nih.gov/pubmed/20421946 http://dx.doi.org/10.1371/journal.pgen.1000922 |
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