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Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
Genetically encoded calcium indicators (GECIs) can be used to image activity in defined neuronal populations. However, current GECIs produce inferior signals compared to synthetic indicators and recording electrodes, precluding detection of low firing rates. We developed a single-wavelength GECI bas...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858873/ https://www.ncbi.nlm.nih.gov/pubmed/19898485 http://dx.doi.org/10.1038/nmeth.1398 |
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author | Tian, Lin Hires, S. Andrew Mao, Tianyi Huber, Daniel Chiappe, M. Eugenia Chalasani, Sreekanth H. Petreanu, Leopoldo Akerboom, Jasper McKinney, Sean A. Schreiter, Eric R. Bargmann, Cornelia I. Jayaraman, Vivek Svoboda, Karel Looger, Loren L. |
author_facet | Tian, Lin Hires, S. Andrew Mao, Tianyi Huber, Daniel Chiappe, M. Eugenia Chalasani, Sreekanth H. Petreanu, Leopoldo Akerboom, Jasper McKinney, Sean A. Schreiter, Eric R. Bargmann, Cornelia I. Jayaraman, Vivek Svoboda, Karel Looger, Loren L. |
author_sort | Tian, Lin |
collection | PubMed |
description | Genetically encoded calcium indicators (GECIs) can be used to image activity in defined neuronal populations. However, current GECIs produce inferior signals compared to synthetic indicators and recording electrodes, precluding detection of low firing rates. We developed a single-wavelength GECI based on GCaMP2 (GCaMP3), with increased baseline fluorescence (3x), dynamic range (3x), and higher affinity for calcium (1.3x). GCaMP3 fluorescence changes triggered by single action potentials were detected in pyramidal cell dendrites, with signal-to-noise ratio and photostability significantly better than GCaMP2, D3cpVenus, and TN-XXL. In Caenorhabditis elegans chemosensory neurons and the Drosophila melanogaster antennal lobe, sensory stimulation-evoked fluorescence responses were significantly enhanced with the new indicator (4–6x). In somatosensory and motor cortical neurons in the intact mouse, GCaMP3 detected calcium transients with amplitudes linearly dependent on action potential number. Long-term imaging in the motor cortex of behaving mice revealed large fluorescence changes in imaged neurons over months. |
format | Text |
id | pubmed-2858873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-28588732010-06-01 Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators Tian, Lin Hires, S. Andrew Mao, Tianyi Huber, Daniel Chiappe, M. Eugenia Chalasani, Sreekanth H. Petreanu, Leopoldo Akerboom, Jasper McKinney, Sean A. Schreiter, Eric R. Bargmann, Cornelia I. Jayaraman, Vivek Svoboda, Karel Looger, Loren L. Nat Methods Article Genetically encoded calcium indicators (GECIs) can be used to image activity in defined neuronal populations. However, current GECIs produce inferior signals compared to synthetic indicators and recording electrodes, precluding detection of low firing rates. We developed a single-wavelength GECI based on GCaMP2 (GCaMP3), with increased baseline fluorescence (3x), dynamic range (3x), and higher affinity for calcium (1.3x). GCaMP3 fluorescence changes triggered by single action potentials were detected in pyramidal cell dendrites, with signal-to-noise ratio and photostability significantly better than GCaMP2, D3cpVenus, and TN-XXL. In Caenorhabditis elegans chemosensory neurons and the Drosophila melanogaster antennal lobe, sensory stimulation-evoked fluorescence responses were significantly enhanced with the new indicator (4–6x). In somatosensory and motor cortical neurons in the intact mouse, GCaMP3 detected calcium transients with amplitudes linearly dependent on action potential number. Long-term imaging in the motor cortex of behaving mice revealed large fluorescence changes in imaged neurons over months. 2009-11-08 2009-12 /pmc/articles/PMC2858873/ /pubmed/19898485 http://dx.doi.org/10.1038/nmeth.1398 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Tian, Lin Hires, S. Andrew Mao, Tianyi Huber, Daniel Chiappe, M. Eugenia Chalasani, Sreekanth H. Petreanu, Leopoldo Akerboom, Jasper McKinney, Sean A. Schreiter, Eric R. Bargmann, Cornelia I. Jayaraman, Vivek Svoboda, Karel Looger, Loren L. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators |
title | Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators |
title_full | Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators |
title_fullStr | Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators |
title_full_unstemmed | Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators |
title_short | Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators |
title_sort | imaging neural activity in worms, flies and mice with improved gcamp calcium indicators |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858873/ https://www.ncbi.nlm.nih.gov/pubmed/19898485 http://dx.doi.org/10.1038/nmeth.1398 |
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