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Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci

Nonsynonymous SNPs (nsSNPs) in DNA repair genes may be important determinants of DNA damage and cancer risk. We applied a set of screening criteria to a large number of nsSNPs and selected a subset of SNPs that were likely candidates for phenotypic effects on DNA double-strand break repair (DSBR). I...

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Detalles Bibliográficos
Autores principales: Markunas, Christina A., Umbach, David M., Xu, Zongli, Taylor, Jack A.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858903/
https://www.ncbi.nlm.nih.gov/pubmed/20445776
http://dx.doi.org/10.1155/2008/387423
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author Markunas, Christina A.
Umbach, David M.
Xu, Zongli
Taylor, Jack A.
author_facet Markunas, Christina A.
Umbach, David M.
Xu, Zongli
Taylor, Jack A.
author_sort Markunas, Christina A.
collection PubMed
description Nonsynonymous SNPs (nsSNPs) in DNA repair genes may be important determinants of DNA damage and cancer risk. We applied a set of screening criteria to a large number of nsSNPs and selected a subset of SNPs that were likely candidates for phenotypic effects on DNA double-strand break repair (DSBR). In order to induce and follow DSBR, we exposed panels of cell lines to gamma irradiation and followed the formation and disappearance of γH2A.X foci over time. All panels of cell lines showed significant increases in number, intensity, and area of foci at both the 1-hour and 3-hour time points. Twenty four hours following exposure, the number of foci returned to preexposure levels in all cell lines, whereas the size and intensity of foci remained significantly elevated. We saw no significant difference in γH2A.X foci between controls and any of the panels of cell lines representing the different nsSNPs.
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spelling pubmed-28589032010-05-05 Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci Markunas, Christina A. Umbach, David M. Xu, Zongli Taylor, Jack A. J Cancer Epidemiol Research Article Nonsynonymous SNPs (nsSNPs) in DNA repair genes may be important determinants of DNA damage and cancer risk. We applied a set of screening criteria to a large number of nsSNPs and selected a subset of SNPs that were likely candidates for phenotypic effects on DNA double-strand break repair (DSBR). In order to induce and follow DSBR, we exposed panels of cell lines to gamma irradiation and followed the formation and disappearance of γH2A.X foci over time. All panels of cell lines showed significant increases in number, intensity, and area of foci at both the 1-hour and 3-hour time points. Twenty four hours following exposure, the number of foci returned to preexposure levels in all cell lines, whereas the size and intensity of foci remained significantly elevated. We saw no significant difference in γH2A.X foci between controls and any of the panels of cell lines representing the different nsSNPs. Hindawi Publishing Corporation 2008 2009-03-12 /pmc/articles/PMC2858903/ /pubmed/20445776 http://dx.doi.org/10.1155/2008/387423 Text en Copyright © 2008 Christina A. Markunas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Markunas, Christina A.
Umbach, David M.
Xu, Zongli
Taylor, Jack A.
Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
title Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
title_full Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
title_fullStr Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
title_full_unstemmed Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
title_short Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-Induced γH2A.X Foci
title_sort assessing candidate gene nssnps for phenotypic differences in double-strand break repair using radiation-induced γh2a.x foci
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858903/
https://www.ncbi.nlm.nih.gov/pubmed/20445776
http://dx.doi.org/10.1155/2008/387423
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