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Site-specific labeling of nucleotides for making RNA for high resolution NMR studies using an E. coli strain disabled in the oxidative pentose phosphate pathway
Escherichia coli (E. coli) is a versatile organism for making nucleotides labeled with stable isotopes ((13)C, (15)N, and/or (2)H) for structural and molecular dynamics characterizations. Growth of a mutant E. coli strain deficient in the pentose phosphate pathway enzyme glucose-6-phosphate dehydrog...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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Springer Netherlands
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2859161/ https://www.ncbi.nlm.nih.gov/pubmed/20309608 http://dx.doi.org/10.1007/s10858-010-9405-0 |