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Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast
The complete synthetic Mycoplasma genitalium genome (∼583 kb) has been assembled and cloned as a circular plasmid in the yeast Saccharomyces cerevisiae. Attempts to engineer the cloned genome by standard genetic methods involving the URA3/5-fluoroorotic acid (5-FOA) counter-selection have shown a hi...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860121/ https://www.ncbi.nlm.nih.gov/pubmed/20228123 http://dx.doi.org/10.1093/nar/gkq099 |