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Evolved orthogonal ribosome purification for in vitro characterization

We developed orthogonal ribosome−mRNA pairs in which the orthogonal ribosome (O-ribosome) specifically translates the orthogonal mRNA and the orthogonal mRNA is not a substrate for cellular ribosomes. O-ribosomes have been used to create new cellular circuits to control gene expression in new ways,...

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Detalles Bibliográficos
Autores principales: Barrett, Oliver P. T., Chin, Jason W.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860124/
https://www.ncbi.nlm.nih.gov/pubmed/20185573
http://dx.doi.org/10.1093/nar/gkq120