Cargando…

Evolved orthogonal ribosome purification for in vitro characterization

We developed orthogonal ribosome−mRNA pairs in which the orthogonal ribosome (O-ribosome) specifically translates the orthogonal mRNA and the orthogonal mRNA is not a substrate for cellular ribosomes. O-ribosomes have been used to create new cellular circuits to control gene expression in new ways,...

Descripción completa

Detalles Bibliográficos
Autores principales:	Barrett, Oliver P. T., Chin, Jason W.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860124/ https://www.ncbi.nlm.nih.gov/pubmed/20185573 http://dx.doi.org/10.1093/nar/gkq120

Ejemplares similares

Engineering synthetic TAL effectors with orthogonal target sites
por: Garg, Abhishek, et al.
Publicado: (2012)

Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA
por: Hughes, Randall A., et al.
Publicado: (2010)

The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
por: Fritz, Brian R., et al.
Publicado: (2014)

Modular control of multiple pathways using engineered orthogonal T7 polymerases
por: Temme, Karsten, et al.
Publicado: (2012)

A modular strategy for engineering orthogonal chimeric RNA transcription regulators
por: Takahashi, Melissa K., et al.
Publicado: (2013)

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation
por: Albayrak, Cem, et al.
Publicado: (2013)

Mutational characterization and mapping of the 70S ribosome active site
por: d’Aquino, Anne E, et al.
Publicado: (2020)

Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction
por: Fritz, Brian R., et al.
Publicado: (2015)

A switch-on mechanism to activate maize ribosome-inactivating protein for targeting HIV-infected cells
por: Law, Sue Ka-Yee, et al.
Publicado: (2010)

Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration
por: Yang, Siqi, et al.
Publicado: (2021)

SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems
por: Ba, Fang, et al.
Publicado: (2022)

Engineering orthogonal synthetic timer circuits based on extracytoplasmic function σ factors
por: Pinto, Daniela, et al.
Publicado: (2018)

From dynamic combinatorial ‘hit’ to lead: in vitro and in vivo activity of compounds targeting the pathogenic RNAs that cause myotonic dystrophy
por: Ofori, Leslie O., et al.
Publicado: (2012)

Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality
por: Chaillou, Santiago, et al.
Publicado: (2022)

Computational design of orthogonal ribosomes
por: Chubiz, Lon M., et al.
Publicado: (2008)

Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro
por: Shimo, Takenori, et al.
Publicado: (2014)

Design, construction and characterization of a set of insulated bacterial promoters
por: Davis, Joseph H., et al.
Publicado: (2011)

Probing the in vitro mechanism of action of cationic lipid/DNA lipoplexes at a nanometric scale
por: Le Bihan, Olivier, et al.
Publicado: (2011)

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
por: Chappell, James, et al.
Publicado: (2013)

Characterization of photophysical and base-mimicking properties of a novel fluorescent adenine analogue in DNA
por: Dierckx, Anke, et al.
Publicado: (2011)

Novel post-synthetic generation, isomeric resolution, and characterization of Fapy-dG within oligodeoxynucleotides: differential anomeric impacts on DNA duplex properties
por: Lukin, Mark, et al.
Publicado: (2011)

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology
por: Huang, Hsin-Ho, et al.
Publicado: (2010)

Synthesis and characterization of 2′-modified-4′-thioRNA: a comprehensive comparison of nuclease stability
por: Takahashi, Mayumi, et al.
Publicado: (2009)

Synthesis and structural characterization of piperazino-modified DNA that favours hybridization towards DNA over RNA
por: Skov, Joan, et al.
Publicado: (2011)

Design and characterization of a dual-mode promoter with activation and repression capability for tuning gene expression in yeast
por: Mazumder, Mostafizur, et al.
Publicado: (2014)

Computationally-guided design and selection of high performing ribosomal active site mutants
por: Kofman, Camila, et al.
Publicado: (2022)

Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli
por: Cress, Brady F., et al.
Publicado: (2016)

In vitro optimization of 2′-OMe-4′-thioribonucleoside–modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle
por: Takahashi, Mayumi, et al.
Publicado: (2013)

Influence of substituent modifications on the binding of 2-amino-1,8-naphthyridines to cytosine opposite an AP site in DNA duplexes: thermodynamic characterization
por: Sato, Yusuke, et al.
Publicado: (2009)

Synthesis and characterization of oligodeoxyribonucleotides containing a site-specifically incorporated N(6)-carboxymethyl-2′-deoxyadenosine or N(4)-carboxymethyl-2′-deoxycytidine
por: Wang, Jianshuang, et al.
Publicado: (2010)

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus
por: Lansing, Felix, et al.
Publicado: (2020)

Systematic design and functional analysis of artificial microRNAs
por: Arroyo, Jason D., et al.
Publicado: (2014)

Programmable cross-ribosome-binding sites to fine-tune the dynamic range of transcription factor-based biosensor
por: Ding, Nana, et al.
Publicado: (2020)

A tunable zinc finger-based framework for Boolean logic computation in mammalian cells
por: Lohmueller, Jason J., et al.
Publicado: (2012)

Rational design of a synthetic mammalian riboswitch as a ligand-responsive -1 ribosomal frame-shifting stimulator
por: Lin, Ya-Hui, et al.
Publicado: (2016)

MegaTevs: single-chain dual nucleases for efficient gene disruption
por: Wolfs, Jason M., et al.
Publicado: (2014)

Greedy reduction of Bacillus subtilis genome yields emergent phenotypes of high resistance to a DNA damaging agent and low evolvability
por: Dervyn, Etienne, et al.
Publicado: (2023)

DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency
por: Conrado, Robert J., et al.
Publicado: (2012)

Premature translation termination mediated non-ER stress induced ATF6 activation by a ligand-dependent ribosomal frameshifting circuit
por: Hsu, Hsiu-Ting, et al.
Publicado: (2022)

Highly fluorescent guanosine mimics for folding and energy transfer studies
por: Dumas, Anaëlle, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_1qpig432oahmtpcncct09saqrr