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Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method
The stable cloning of giant DNA is a necessary process in the production of recombinant/synthetic genomes. Handling DNA molecules in test tubes becomes increasingly difficult as their size increases, particularly above 100 kb. The need to prepare such large DNA molecules in a regular manner has limi...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860128/ https://www.ncbi.nlm.nih.gov/pubmed/20308163 http://dx.doi.org/10.1093/nar/gkq142 |