Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

We have achieved the ability to synthesize thousands of unique, long oligonucleotides (150mers) in fmol amounts using parallel synthesis of DNA on microarrays. The sequence accuracy of the oligonucleotides in such large-scale syntheses has been limited by the yields and side reactions of the DNA syn...

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Detalles Bibliográficos
Autores principales: LeProust, Emily M., Peck, Bill J., Spirin, Konstantin, McCuen, Heather Brummel, Moore, Bridget, Namsaraev, Eugeni, Caruthers, Marvin H.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Synthetic Biology and Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860131/
https://www.ncbi.nlm.nih.gov/pubmed/20308161
http://dx.doi.org/10.1093/nar/gkq163

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860131/
https://www.ncbi.nlm.nih.gov/pubmed/20308161
http://dx.doi.org/10.1093/nar/gkq163

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