A streptavidin variant with slower biotin dissociation and increased mechanostability

Streptavidin binds biotin-conjugates with exceptional stability, but dissociation does occur and can be limiting in imaging, DNA amplification, and nanotechnology. We identified a mutant streptavidin, which we call traptavidin, showing ~10-fold slower biotin off-rate, increased mechanical strength,...

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Detalles Bibliográficos
Autores principales: Chivers, Claire E., Crozat, Estelle, Chu, Calvin, Moy, Vincent T., Sherratt, David J., Howarth, Mark
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862113/
https://www.ncbi.nlm.nih.gov/pubmed/20383133
http://dx.doi.org/10.1038/nmeth.1450

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862113/
https://www.ncbi.nlm.nih.gov/pubmed/20383133
http://dx.doi.org/10.1038/nmeth.1450

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