Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host

BACKGROUND: Escherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence of resident clones via recombineering. BAC and fosmid clones are highly suitable for modification by recombineering but, because t...

Descripción completa

Detalles Bibliográficos
Autores principales: Westenberg, Marcel, Bamps, Sophie, Soedling, Helen, Hope, Ian A, Dolphin, Colin T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864197/
https://www.ncbi.nlm.nih.gov/pubmed/20350301
http://dx.doi.org/10.1186/1472-6750-10-27

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864197/
https://www.ncbi.nlm.nih.gov/pubmed/20350301
http://dx.doi.org/10.1186/1472-6750-10-27

Ejemplares similares