Cargando…

Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host

BACKGROUND: Escherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence of resident clones via recombineering. BAC and fosmid clones are highly suitable for modification by recombineering but, because t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Westenberg, Marcel, Bamps, Sophie, Soedling, Helen, Hope, Ian A, Dolphin, Colin T
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864197/ https://www.ncbi.nlm.nih.gov/pubmed/20350301 http://dx.doi.org/10.1186/1472-6750-10-27

Ejemplares similares

Lambda Red-mediated Recombineering in the Attaching and Effacing Pathogen Escherichia albertii
por: Egan, Marisa, et al.
Publicado: (2016)

A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones
por: Hirani, Nisha, et al.
Publicado: (2013)

Counter-selection recombineering of the baculovirus genome: a strategy for seamless modification of repeat-containing BACs
por: Westenberg, Marcel, et al.
Publicado: (2010)

Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes
por: Serra-Moreno, Ruth, et al.
Publicado: (2006)

Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
por: Murphy, Kenan C, et al.
Publicado: (2003)

Seamless replacement of Autographa californica multiple nucleopolyhedrovirus gp64 with each of five novel type II alphabaculovirus fusion sequences generates pseudotyped virus that fails to transduce mammalian cells
por: Westenberg, Marcel, et al.
Publicado: (2012)

Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa
por: Lesic, Biliana, et al.
Publicado: (2008)

Are Only p-Values Less Than 0.05 Significant? A p-Value Greater Than 0.05 Is Also Significant!
por: Kwak, Sanggyu
Publicado: (2023)

Statistical significance: p value, 0.05 threshold, and applications to radiomics—reasons for a conservative approach
por: Di Leo, Giovanni, et al.
Publicado: (2020)

Bacteriophage recombineering in the lytic state using the lambda red recombinases
por: Fehér, Tamás, et al.
Publicado: (2012)

The significance of alternative transcripts for Caenorhabditis elegans transcription factor genes, based on expression pattern analysis
por: Craig, Hannah L, et al.
Publicado: (2013)

Involvement of Escherichia coli DNA Replication Proteins in Phage Lambda Red-Mediated Homologous Recombination
por: Poteete, Anthony R.
Publicado: (2013)

Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
por: Schmeisser, Falko, et al.
Publicado: (2007)

Selection and characterization of a promoter for expression of single-copy recombinant genes in Gram-positive bacteria
por: Provvedi, Roberta, et al.
Publicado: (2005)

Cancrum Oris
Publicado: (1887)

Cancrum Oris
Publicado: (1848)

Cancrum Oris
Publicado: (1852)

Cancrum Oris
por: Kittredge, C. S.
Publicado: (1872)

Cancrum Oris
por: Shaw, J. P.
Publicado: (1896)

Cancrum Oris
por: Gray, James
Publicado: (1856)

On Cancrum Oris
por: Keiller, Alexander
Publicado: (1862)

Cancrum Oris
por: Duke, B. F.
Publicado: (1881)

Cancrum Oris
por: Fleming, W. P.
Publicado: (1891)

Cancrum Oris
por: Brackett, Charles
Publicado: (1860)

CopyRighter: a rapid tool for improving the accuracy of microbial community profiles through lineage-specific gene copy number correction
por: Angly, Florent E, et al.
Publicado: (2014)

Development of copy number assays for detection and surveillance of piperaquine resistance associated plasmepsin 2/3 copy number variation in Plasmodium falciparum
por: Ansbro, Megan R., et al.
Publicado: (2020)

Case of Cancrum Oris
por: Elder, George
Publicado: (1893)

Case of Atresia Oris
por: Cayley, H.
Publicado: (1872)

Cases of Cancrum Oris
por: Levison, J. L.
Publicado: (1851)

Case of Cancrum Oris
por: Elder, George
Publicado: (1893)

Paper on Cancrum Oris
por: Kittredge, C. S.
Publicado: (1872)

Cancrum Oris—Baptisia
por: James, Walter M.
Publicado: (1884)

Cases of Cancrum Oris
por: Brackett, Charles
Publicado: (1852)

Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus
por: García-Ríos, Estéfani, et al.
Publicado: (2021)

Copy number variation analysis based on AluScan sequences
por: Yang, Jian-Feng, et al.
Publicado: (2014)

Kappa and lambda light chain mRNA in situ hybridization compared to flow cytometry and immunohistochemistry in B cell lymphomas
por: Rimsza, Lisa M, et al.
Publicado: (2014)

An Eye Simulation for Training in the Use of Ophthalmologic Equipment and Corneal Foreign Body Removal Techniques
por: Thompson, Linda Brindley
Publicado: (2020)

Identification of oriT and a recombination hot spot in the IncA/C plasmid backbone
por: Hegyi, Anna, et al.
Publicado: (2017)

Identification of Medium-Sized Copy Number Alterations in Whole-Genome Sequencing
por: Ozer, Hatice Gulcin, et al.
Publicado: (2015)

Noise cancellation using total variation for copy number variation detection
por: Zare, Fatima, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_amaa3oe593hgbbpivsnajqh35u