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High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function

BACKGROUND: Cultured spinal motor neurons are a valuable tool to study basic mechanisms of development, axon growth and pathfinding, and, importantly, to analyze the pathomechanisms underlying motor neuron diseases. However, the application of this cell culture model is limited by the lack of effici...

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Detalles Bibliográficos
Autores principales: Fallini, Claudia, Bassell, Gary J, Rossoll, Wilfried
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2867961/
https://www.ncbi.nlm.nih.gov/pubmed/20406490
http://dx.doi.org/10.1186/1750-1326-5-17