Restriction Site Extension PCR: A Novel Method for High-Throughput Characterization of Tagged DNA Fragments and Genome Walking

BACKGROUND: Insertion mutant isolation and characterization are extremely valuable for linking genes to physiological function. Once an insertion mutant phenotype is identified, the challenge is to isolate the responsible gene. Multiple strategies have been employed to isolate unknown genomic DNA th...

Descripción completa

Detalles Bibliográficos
Autores principales: Ji, Jiabing, Braam, Janet
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868030/
https://www.ncbi.nlm.nih.gov/pubmed/20485508
http://dx.doi.org/10.1371/journal.pone.0010577

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868030/
https://www.ncbi.nlm.nih.gov/pubmed/20485508
http://dx.doi.org/10.1371/journal.pone.0010577

Ejemplares similares