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Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors

Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine–glycine (FG)-rich repeat...

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Detalles Bibliográficos
Autores principales: Eisele, Nico B, Frey, Steffen, Piehler, Jacob, Görlich, Dirk, Richter, Ralf P
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868541/
https://www.ncbi.nlm.nih.gov/pubmed/20379223
http://dx.doi.org/10.1038/embor.2010.34
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author Eisele, Nico B
Frey, Steffen
Piehler, Jacob
Görlich, Dirk
Richter, Ralf P
author_facet Eisele, Nico B
Frey, Steffen
Piehler, Jacob
Görlich, Dirk
Richter, Ralf P
author_sort Eisele, Nico B
collection PubMed
description Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine–glycine (FG)-rich repeats (FG repeat domains), in a way that is at present poorly understood. We have developed ultrathin FG domain films that reproduce the mode of attachment and the density of FG repeats in NPCs, and that exhibit a thickness that corresponds to the nanoscopic dimensions of the native permeability barrier. By using a combination of biophysical characterization techniques, we quantified the binding of nuclear transport receptors (NTRs) to such FG domain films and analysed how this binding affects the swelling behaviour and mechanical properties of the films. The results extend our understanding of the interaction of FG domain assemblies with NTRs and contribute important information to refine the model of transport across the permeability barrier.
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spelling pubmed-28685412010-05-20 Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors Eisele, Nico B Frey, Steffen Piehler, Jacob Görlich, Dirk Richter, Ralf P EMBO Rep Scientific Reports Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine–glycine (FG)-rich repeats (FG repeat domains), in a way that is at present poorly understood. We have developed ultrathin FG domain films that reproduce the mode of attachment and the density of FG repeats in NPCs, and that exhibit a thickness that corresponds to the nanoscopic dimensions of the native permeability barrier. By using a combination of biophysical characterization techniques, we quantified the binding of nuclear transport receptors (NTRs) to such FG domain films and analysed how this binding affects the swelling behaviour and mechanical properties of the films. The results extend our understanding of the interaction of FG domain assemblies with NTRs and contribute important information to refine the model of transport across the permeability barrier. Nature Publishing Group 2010-05 2010-04-09 /pmc/articles/PMC2868541/ /pubmed/20379223 http://dx.doi.org/10.1038/embor.2010.34 Text en Copyright © 2010, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.
spellingShingle Scientific Reports
Eisele, Nico B
Frey, Steffen
Piehler, Jacob
Görlich, Dirk
Richter, Ralf P
Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
title Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
title_full Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
title_fullStr Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
title_full_unstemmed Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
title_short Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
title_sort ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
topic Scientific Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868541/
https://www.ncbi.nlm.nih.gov/pubmed/20379223
http://dx.doi.org/10.1038/embor.2010.34
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