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Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors
Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine–glycine (FG)-rich repeat...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868541/ https://www.ncbi.nlm.nih.gov/pubmed/20379223 http://dx.doi.org/10.1038/embor.2010.34 |
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author | Eisele, Nico B Frey, Steffen Piehler, Jacob Görlich, Dirk Richter, Ralf P |
author_facet | Eisele, Nico B Frey, Steffen Piehler, Jacob Görlich, Dirk Richter, Ralf P |
author_sort | Eisele, Nico B |
collection | PubMed |
description | Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine–glycine (FG)-rich repeats (FG repeat domains), in a way that is at present poorly understood. We have developed ultrathin FG domain films that reproduce the mode of attachment and the density of FG repeats in NPCs, and that exhibit a thickness that corresponds to the nanoscopic dimensions of the native permeability barrier. By using a combination of biophysical characterization techniques, we quantified the binding of nuclear transport receptors (NTRs) to such FG domain films and analysed how this binding affects the swelling behaviour and mechanical properties of the films. The results extend our understanding of the interaction of FG domain assemblies with NTRs and contribute important information to refine the model of transport across the permeability barrier. |
format | Text |
id | pubmed-2868541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-28685412010-05-20 Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors Eisele, Nico B Frey, Steffen Piehler, Jacob Görlich, Dirk Richter, Ralf P EMBO Rep Scientific Reports Nuclear pore complexes (NPCs) are highly selective gates that mediate the exchange of all proteins and nucleic acids between the cytoplasm and the nucleus. Their selectivity relies on a supramolecular assembly of natively unfolded nucleoporin domains containing phenylalanine–glycine (FG)-rich repeats (FG repeat domains), in a way that is at present poorly understood. We have developed ultrathin FG domain films that reproduce the mode of attachment and the density of FG repeats in NPCs, and that exhibit a thickness that corresponds to the nanoscopic dimensions of the native permeability barrier. By using a combination of biophysical characterization techniques, we quantified the binding of nuclear transport receptors (NTRs) to such FG domain films and analysed how this binding affects the swelling behaviour and mechanical properties of the films. The results extend our understanding of the interaction of FG domain assemblies with NTRs and contribute important information to refine the model of transport across the permeability barrier. Nature Publishing Group 2010-05 2010-04-09 /pmc/articles/PMC2868541/ /pubmed/20379223 http://dx.doi.org/10.1038/embor.2010.34 Text en Copyright © 2010, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission. |
spellingShingle | Scientific Reports Eisele, Nico B Frey, Steffen Piehler, Jacob Görlich, Dirk Richter, Ralf P Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
title | Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
title_full | Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
title_fullStr | Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
title_full_unstemmed | Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
title_short | Ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
title_sort | ultrathin nucleoporin phenylalanine–glycine repeat films and their interaction with nuclear transport receptors |
topic | Scientific Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868541/ https://www.ncbi.nlm.nih.gov/pubmed/20379223 http://dx.doi.org/10.1038/embor.2010.34 |
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