Co-expression of Skp and FkpA chaperones improves cell viability and alters the global expression of stress response genes during scFvD1.3 production

BACKGROUND: The overexpression of scFv antibody fragments in the periplasmic space of Escherichia coli frequently results in extensive protein misfolding and loss of cell viability. Although protein folding factors such as Skp and FkpA are often exploited to restore the solubility and functionality...

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Detalles Bibliográficos
Autores principales: Ow, Dave Siak-Wei, Lim, Denis Yong-Xiang, Morin Nissom, Peter, Camattari, Andrea, Wong, Victor Vai-Tak
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868799/
https://www.ncbi.nlm.nih.gov/pubmed/20388215
http://dx.doi.org/10.1186/1475-2859-9-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868799/
https://www.ncbi.nlm.nih.gov/pubmed/20388215
http://dx.doi.org/10.1186/1475-2859-9-22

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